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Novus Biologicals il 1ra
H/CS‐MOS hydrogels regulate the recruitment and polarization of macrophages. a–c) Flow cytometric analysis showing the proportion of the macrophages among the recruited cells (a) and the proportion of the M2 macrophages among all the macrophages (b) along with their quantitative comparison (c) ( n = 3). d–f) Flow cytometry analysis of the polarization of the macrophages, indicated by CD197 (M1 phenotype, d) and CD206 (M2 phenotype, e), along with their quantitative comparison (f) ( n = 3). g) Flow cytometry analysis showing the polarization shift of M1 macrophages (induced with LPS and IFN‐γ) cultured on the different scaffolds, along with their quantitative comparison ( n = 3). h,i) Western blot analysis of protein levels of CD206, CD44, pro‐inflammatory factors (IFN‐γ and TNF‐α), anti‐inflammatory factor <t>(IL‐1Ra),</t> and Arg‐1, with quantification normalized to the HA group ( n = 3). j) Quantitative analysis of inflammatory cytokine levels of IL‐6, IL‐1β, TNF‐α, IL‐10 and TGF‐β ( n = 3). All data are presented as mean ± SD. (The p values were determined using unpaired two‐tailed t tests for two‐group comparisons and one‐way ANOVA followed by Tukey's post hoc test for multiple‐group comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, # indicates a comparison with the HA group within the same protein experimental group.
Il 1ra, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nbp1+32568/IL-1ra%2FIL-1F3%2FIL1RN+Antibody/pmc12893307-351-34-36
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90
Novus Biologicals nbp1 32568
H/CS‐MOS hydrogels regulate the recruitment and polarization of macrophages. a–c) Flow cytometric analysis showing the proportion of the macrophages among the recruited cells (a) and the proportion of the M2 macrophages among all the macrophages (b) along with their quantitative comparison (c) ( n = 3). d–f) Flow cytometry analysis of the polarization of the macrophages, indicated by CD197 (M1 phenotype, d) and CD206 (M2 phenotype, e), along with their quantitative comparison (f) ( n = 3). g) Flow cytometry analysis showing the polarization shift of M1 macrophages (induced with LPS and IFN‐γ) cultured on the different scaffolds, along with their quantitative comparison ( n = 3). h,i) Western blot analysis of protein levels of CD206, CD44, pro‐inflammatory factors (IFN‐γ and TNF‐α), anti‐inflammatory factor <t>(IL‐1Ra),</t> and Arg‐1, with quantification normalized to the HA group ( n = 3). j) Quantitative analysis of inflammatory cytokine levels of IL‐6, IL‐1β, TNF‐α, IL‐10 and TGF‐β ( n = 3). All data are presented as mean ± SD. (The p values were determined using unpaired two‐tailed t tests for two‐group comparisons and one‐way ANOVA followed by Tukey's post hoc test for multiple‐group comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, # indicates a comparison with the HA group within the same protein experimental group.
Nbp1 32568, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nbp1+32568/IL-1ra%2FIL-1F3%2FIL1RN+Antibody/pmc05860832-112-50-54
Average 90 stars, based on 1 article reviews
nbp1 32568 - by Bioz Stars, 2026-09
90/100 stars
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H/CS‐MOS hydrogels regulate the recruitment and polarization of macrophages. a–c) Flow cytometric analysis showing the proportion of the macrophages among the recruited cells (a) and the proportion of the M2 macrophages among all the macrophages (b) along with their quantitative comparison (c) ( n = 3). d–f) Flow cytometry analysis of the polarization of the macrophages, indicated by CD197 (M1 phenotype, d) and CD206 (M2 phenotype, e), along with their quantitative comparison (f) ( n = 3). g) Flow cytometry analysis showing the polarization shift of M1 macrophages (induced with LPS and IFN‐γ) cultured on the different scaffolds, along with their quantitative comparison ( n = 3). h,i) Western blot analysis of protein levels of CD206, CD44, pro‐inflammatory factors (IFN‐γ and TNF‐α), anti‐inflammatory factor (IL‐1Ra), and Arg‐1, with quantification normalized to the HA group ( n = 3). j) Quantitative analysis of inflammatory cytokine levels of IL‐6, IL‐1β, TNF‐α, IL‐10 and TGF‐β ( n = 3). All data are presented as mean ± SD. (The p values were determined using unpaired two‐tailed t tests for two‐group comparisons and one‐way ANOVA followed by Tukey's post hoc test for multiple‐group comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, # indicates a comparison with the HA group within the same protein experimental group.

Journal: Small Methods

Article Title: Mannose Oligosaccharide‐Conjugated In Situ Pore‐Forming Injectable Hydrogels for Rheumatoid Arthritis Treatment by Reprogramming Macrophage Extracellular Vesicles

doi: 10.1002/smtd.202500605

Figure Lengend Snippet: H/CS‐MOS hydrogels regulate the recruitment and polarization of macrophages. a–c) Flow cytometric analysis showing the proportion of the macrophages among the recruited cells (a) and the proportion of the M2 macrophages among all the macrophages (b) along with their quantitative comparison (c) ( n = 3). d–f) Flow cytometry analysis of the polarization of the macrophages, indicated by CD197 (M1 phenotype, d) and CD206 (M2 phenotype, e), along with their quantitative comparison (f) ( n = 3). g) Flow cytometry analysis showing the polarization shift of M1 macrophages (induced with LPS and IFN‐γ) cultured on the different scaffolds, along with their quantitative comparison ( n = 3). h,i) Western blot analysis of protein levels of CD206, CD44, pro‐inflammatory factors (IFN‐γ and TNF‐α), anti‐inflammatory factor (IL‐1Ra), and Arg‐1, with quantification normalized to the HA group ( n = 3). j) Quantitative analysis of inflammatory cytokine levels of IL‐6, IL‐1β, TNF‐α, IL‐10 and TGF‐β ( n = 3). All data are presented as mean ± SD. (The p values were determined using unpaired two‐tailed t tests for two‐group comparisons and one‐way ANOVA followed by Tukey's post hoc test for multiple‐group comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # p < 0.05, ## p < 0.01, ### p < 0.001, # indicates a comparison with the HA group within the same protein experimental group.

Article Snippet: The membrane pieces were probed overnight at 4 °C with primary antibodies against GAPDH (#2118, Cell Signaling Technology, CST, Danvers, MA, USA), CD206 (ab64693, Abcam), CD44 (ab157107, Abcam), IFN‐γ (ab198801, Abcam), TNF‐α (ab307164, Abcam), IL‐1Ra (NBP1‐32568, Novus Biologicals, Littleton, CO, USA) and Arg‐1 (#93668, CST).

Techniques: Comparison, Flow Cytometry, Cell Culture, Western Blot, Two Tailed Test